The 2-Minute Rule for thr77 login
The 2-Minute Rule for thr77 login
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and thus could contribute to tumorigenesis. This protein was identified to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic function. Cyclin E sorts a complex with and capabilities as being a regulatory get more info subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress by way of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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